Part:BBa_K4165233

His - UBE2N

This biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165075), His 6-His tag (BBa_K4165020), UBE2N (BBa_K4165011), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2N coding sequence to serve in the purification using NI-NTA column.

Usage and Biology

This part encodes for the UBE2N enzyme which is a member of the E2 ubiquitin-conjugating enzyme family. It acts as the second enzyme in the ubiquitination cascade to receive the thio-esterified ubiquitin from the E1 active site. it will bind to the His tag For the purification of the protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 328
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Mathematical Modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.

                    Figure 1. this figure shows the results from the transcription and translation code showing the 
                                     variation of mRNA and protein concentrations with time.